{"id":55014,"date":"2025-07-01T00:00:00","date_gmt":"2025-07-01T00:00:00","guid":{"rendered":"https:\/\/ainia.endesarrollo.pro\/transparencia\/vbnc-pathogens\/"},"modified":"2025-07-01T00:00:00","modified_gmt":"2025-07-01T00:00:00","slug":"vbnc-pathogens","status":"publish","type":"transparencia","link":"https:\/\/ainia.endesarrollo.pro\/en\/transparencia\/vbnc-pathogens\/","title":{"rendered":"VBNC-PATHOGENS"},"content":{"rendered":"<p>&nbsp;<\/p>\n<h2>Objective<\/h2>\n<p><span style=\"font-weight: 400;\">The general objective of VBNC-PATHOGENS is to design, develop and validate an innovative and robust set of advanced methodologies that enable the<\/span> <b>precise and early detection of foodborne pathogens in a viable but non-culturable (VBNC)<\/b> <b>physiological state, directly from food matrices, with the aim of minimising their entry into the food chain.<\/b> <span style=\"font-weight: 400;\">This project seeks not only to overcome the limitations of traditional culture methods, which are unable to identify this bacterial state, but also to establish a scientific and technological reference framework that integrates current knowledge on the mechanisms of induction and reactivation of the VBNC state, the application of molecular techniques and advanced methodologies, and the validation of the methods developed against ISO standards.<\/span><\/p>\n<p>&nbsp;<\/p>\n<h2>Activities<\/h2>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Prospective study of the current state of detection of foodborne pathogens in the viable but non-culturable (VBNC) state.<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Development of methodologies for obtaining foodborne pathogens such as Salmonella spp. and Listeria monocytogenes in the VBNC state. <\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Analysis of VBNC cells using molecular biology techniques (v-qPCR, RT-qPCR).<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Determination of the VBNC state using high-throughput sequencing technologies.<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Analysis of VBNC by flow cytometry.<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Validation of the techniques developed.<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Coordination and management.<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Dissemination and communication.<\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\">Technology transfer and promotion of results.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\">\n<h2>Dissemination\/Transfer<\/h2>\n<p><a href=\"https:\/\/www.ainia.com\/wp-content\/uploads\/2021\/09\/05_25_26_VBNC-PATHOGENS.pdf\" target=\"_blank\" rel=\"noopener\">VBNC-PATHOGENS poster <\/a><\/p>\n<h2>Press releases<\/h2>\n<p><a href=\"https:\/\/www.ainia.com\/ainia-news\/ainia-impulsa-11-nuevos-proyectos-id-con-respaldo-ivace-feder\/\" target=\"_blank\" rel=\"noopener\">AINIA launches 11 new R&#038;D&#038;I projects with the support of IVACE-FEDER<\/a><\/p>\n<h2>Outreach articles<\/h2>\n<p><a href=\"https:\/\/www.ainia.com\/ainia-news\/deteccion-bacterias-patogenas-estado-viable-no-cultivable-vbnc-industria-alimentaria\/\" target=\"_blank\" rel=\"noopener\">Challenges in detecting pathogenic bacteria in a viable but non-culturable (VBNC) state in the food industry<\/a><\/p>\n<p>&nbsp;<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone wp-image-180198\" src=\"https:\/\/ainia.endesarrollo.pro\/wp-content\/uploads\/IVACEUEdeclaracion-cs-negro-1-scaled-1.png\" alt=\"New IVACE FEDER 2025 logo\" width=\"701\" height=\"75\"><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Development of tools for the assessment and analysis of foodborne pathogenic microorganisms in a viable but non-culturable (VBNC) state<\/p>\n","protected":false},"featured_media":55015,"template":"","meta":{"_acf_changed":false,"footnotes":""},"transparencia_ainia_cat":[236],"transparencia_ainia_tag":[],"class_list":["post-55014","transparencia","type-transparencia","status-publish","has-post-thumbnail","hentry","transparencia_ainia_cat-subsidies-public-funding"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - 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